ATF-1 Antibody #25177
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
ATF-1 Antibody recognizes endogenous levels of total ATF-1 protein. This antibody may detect bands of unknown identity at 130-150 kDa.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human ATF-1 protein. Antibodies are purified by peptide affinity chromatography.
Background
Activating transcription factor 1 (ATF-1) is a bZIP transcription factor that is a member of the cyclic AMP (cAMP)-response element-binding protein (CREB) family. Like other members of the CREB family, ATF-1 mediates signals from numerous physiological stimuli and activates target genes through cAMP-response elements, resulting in the regulation of a broad array of cellular responses. ATF-1 can be phosphorylated at Ser63 by protein kinase A (PKA), calmodulin-dependent protein kinase (CaMK), and cyclin-dependent kinase 3 (CDK3), which recruits CBP/p300 and enhances transcriptional activity (1-3). ATF-1 expression levels have been found to be elevated in lymphomas and metastatic melanoma cells, where it contributes to tumor growth (4,5). The gene fusion of ATF-1 with EWSR1, which encodes for the EWSR1-ATF1 oncoprotein, is also implicated in a variety of cancers, most notably in clear cell sarcoma (CCS) (6).
Limited Uses
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