GIRK2 (D6C2T) Rabbit mAb #25797
- WB
- IP
Supporting Data
REACTIVITY | H M R |
SENSITIVITY | Endogenous |
MW (kDa) | 46 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg46 of human GIRK2 protein.
Background
GIRK2 is a member of G protein-coupled inwardly rectifying potassium channel family proteins (GIRKs). GIRK family proteins allow potassium to flow into the cell and therefore control cellular excitability in the central nervous system, heart, and pancreas (1-4). Activation of most GIRK channels requires heterologous subunit assembly and the presence of ATP (5-7). GIRK2 is abundantly expressed in the brain, where it is involved in pain perception. It is also required for peripheral opioid-mediated analgesia (8). Additionally GIRK2 localizes to pancreatic β cells and regulates insulin secretion (9,10). Mutations in the KCNJ6 gene encoding GIRK2 are associated with Keppen-Lubinsky Syndrome, a rare disease characterized by severe developmental delay, facial dysmorphism, and intellectual disability (11).
- Lesage, F. et al. (1995) J Biol Chem 270, 28660-7.
- Kubo, Y. et al. (1993) Nature 364, 802-6.
- Krapivinsky, G. et al. (1995) Nature 374, 135-41.
- Isomoto, S. et al. (1997) Jpn J Physiol 47, 11-39.
- Duprat, F. et al. (1995) Biochem Biophys Res Commun 212, 657-63.
- Corey, S. and Clapham, D.E. (1998) J Biol Chem 273, 27499-504.
- Han, J. et al. (2003) J Physiol 550, 693-706.
- Nockemann, D. et al. (2013) EMBO Mol Med 5, 1263-77.
- Ferrer, J. et al. (1995) J Biol Chem 270, 26086-91.
- Sakura, H. et al. (1995) FEBS Lett 367, 193-7.
- Masotti, A. et al. (2015) Am J Hum Genet 96, 295-300.
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