GIT-1 Antibody #2919
- WB
- IP
Supporting Data
| REACTIVITY | H M R Hm Mk B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 95 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Hm-Hamster
- Mk-Monkey
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
GIT-1 Antibody recognizes endogenous levels of total GIT-1 protein.
Species Reactivity:
Human, Mouse, Rat, Hamster, Monkey, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human GIT-1. Antibodies are purified using peptide affinity chromatography.
Background
G protein-coupled receptor (GPCR) kinase interacting proteins 1 and 2 (GIT1 and GIT2) are highly conserved, ubiquitous scaffold proteins involved in localized signaling to help regulate focal contact assembly and cytoskeletal dynamics. GIT proteins contain multiple interaction domains that allow interaction with small GTPases (including ARF, Rac, and cdc42), kinases (such as PAK and MEK), the Rho family GEF PIX, and the focal adhesion protein paxillin (reviewed in 1). GIT1 is localized to focal adhesions, cytoplasmic complexes and membrane protrusions, and regulates cell protrusion formation and migration (2). GIT1 has also been implicated in neuronal functions including synapse formation (3) and the pathology of Huntington's disease (HD) (4). HD is a genetic neurodegenerative condition involving a mutation in the htt gene encoding the protein huntingtin. Huntingtin is ubiquitinated and degraded in human HD brains (5). Huntingtin interacts directly with GIT1, causing enhanced proteolysis and indicating that GIT1 distribution and function may contribute to HD pathology (4).
Limited Uses
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