GST-Tag (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate) #3368
- IF
- F
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a |
Application Key:
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:50 - 1:100 |
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
GST-Tag (26H1) Mouse mAb (Alexa Fluor® 488 Conjugate) detects transfected glutathione S-transferase (GST) fusion proteins. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with a GST fusion protein. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio 2-6.
Background
Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.
Glutathione S-transferase (GST) is a widely used fusion partner, since it provides both an easily detectable tag and a simple purification process with little effect on the biological function of the protein of interest. Numerous vectors containing GST-Tag have been developed for both prokaryotic and eukaryotic systems over the past decade (1-3).
Limited Uses
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