PC2 (D1E1S) XP® Rabbit mAb (BSA and Azide Free) #33990
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 65-75 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
This formulation is ideal for use with technologies requiring specialized or custom antibody labeling, including fluorophores, metals, lanthanides, and oligonucleotides. It is not recommended for ChIP, ChIP-seq, CUT&RUN or CUT&Tag assays. If you require a carrier free formulation for chromatin profiling, please contact us. Optimal dilutions/concentrations should be determined by the end user.
Formulation
Storage
Specificity / Sensitivity
PC2 (D1E1S) XP® Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of total PC2 protein.
Species Reactivity:
Human, Mouse, Rat
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Xenopus, Bovine, Horse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro194 of human PC2 protein.
Background
The proprotein convertases (PCs) are enzymes that activate precursor proteins through proteolytic cleavage within the secretory pathway. PCs comprise several enzymes that are basic amino acid-specific proteinases (furin, PC1/3, PC2, PC4, PACE4, PC5/6, and PC7), as well as nonbasic amino acid convertases (S1P and PC9) (1). PCs have a common structure that includes an N-terminal signal peptide for secretory pathway targeting; a pro-domain that is thought to act as an intramolecular chaperone; a catalytic domain containing the active site; a P-domain that contributes to the overall folding of the enzyme by regulating stability, calcium-, and pH-dependence; and a C-terminal domain that interacts with the membrane (2). PCs act in a tissue- and substrate-specific fashion to generate an array of bioactive peptides and proteins from precursors, both in the brain and the periphery (3).
Unlike what is observed with furin whose gene invalidation is lethal, inactivation of mouse PC2 by the introduction of a neomycin resistance gene into the third exon of the PCSK2 gene does not alter mouse viability (4). PC2 inactivation leads to alteration of the pancreatic islet cells, in agreement with the involvment of PC2 in the conversion of pro-insulin and pro-glucagon (5). PC2 is also responsible for the processing of several neuroendocrine peptide precursors such as pro-CCK, POMC, and neurotensin (6).
- Scamuffa, N. et al. (2006) FASEB J 20, 1954-63.
- Fugère, M. and Day, R. (2005) Trends Pharmacol Sci 26, 294-301.
- Seidah, N.G. and Chrétien, M. (1999) Brain Res 848, 45-62.
- Rouillé, Y. et al. (1995) Front Neuroendocrinol 16, 322-61.
- Steiner, D.F. et al. (1996) Diabetes Metab 22, 94-104.
- Scamuffa, N. et al. (2006) FASEB J 20, 1954-63.
- Moffett, R.C. et al. (2014) PLoS One 9, e96863.
Limited Uses
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