TIM-3 (D5D5R™) & CO-0010-750 SignalStar™ Oligo-Antibody Pair #34802
- IHC
Order Information # 34802
This product is not sold separately. Please see the SignalStar™ Multiplex IHC Panel Builder Tool for ordering information.
Product Information
Product Usage Information
Application | Dilution |
---|---|
SignalStar™ Leica Bond | 1:50 - 1:200 |
SignalStar™ Manual | 1:50 - 1:200 |
Storage
Product Description
SignalStar Oligo-Antibody Pairs are compatible with the SignalStar Multiplex IHC Buffer Kits for use in fluorescent multiplex imaging experiments. This product includes the oligo-conjugated antibodies and complementary oligos required for labeling your target protein on up to 10 slides. SignalStar Multiplex IHC Buffer Kits are required to amplify and image the target signal. Multiple oligo-antibody pairs can be conveniently combined into a multiplex panel using the SignalStar Multiplex IHC Panel Builder. SignalStar Multiplex IHC Kits & Reagents are not compatible with all of Cell Signaling Technology® products and protocols that are recommended for use in immunohistochemical assays.
Protocol
Specificity / Sensitivity
TIM-3 (D5D5R™) XP® Rabbit mAb (SignalStar™ Conjugate 0010) recognizes endogenous levels of total TIM-3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human TIM-3 protein.
Background
T cell Ig- and mucin-domain-containing molecules (TIMs) are a family of transmembrane proteins expressed by various immune cells. TIM-3 is an inhibitory molecule that is induced following T cell activation (1-3 ). TIM-3 is expressed by exhausted T cells in the settings of chronic infection and cancer (4,5), and tumor-infiltrating T cells that coexpress PD-1 and TIM-3 exhibit the most severe exhausted phenotype (5). Tumor-infiltrating dendritic cells (DCs) also express TIM-3. TIM-3 expression on DCs was found to suppress innate immunity by reducing the immunogenicity of nucleic acids released by dying tumor cells (6). Research studies show that heterodimerization of TIM-3 with CEACAM-1 is critical for the inhibitory function of TIM-3, and co-blockade of TIM-3 and CEACAM-1 enhanced anti-tumor responses in a mouse model of colorectal cancer (7). In addition, blockade of TIM-3 in mouse models of autoimmunity enhanced the severity of disease (1). Finally, binding of Galectin-9 to TIM-3 expressed by Th1 cells induces T cell death (8).
- Monney, L. et al. (2002) Nature 415, 536-41.
- Sánchez-Fueyo, A. et al. (2003) Nat Immunol 4, 1093-101.
- Sabatos, C.A. et al. (2003) Nat Immunol 4, 1102-10.
- Jones, R.B. et al. (2008) J Exp Med 205, 2763-79.
- Sakuishi, K. et al. (2010) J Exp Med 207, 2187-94.
- Chiba, S. et al. (2012) Nat Immunol 13, 832-42.
- Huang, Y.H. et al. (2015) Nature 517, 386-90.
- Zhu, C. et al. (2005) Nat Immunol 6, 1245-52.
Limited Uses
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