LARS (D7Q4Q) Rabbit mAb #35509
- WB
- IP
Supporting Data
REACTIVITY | H |
SENSITIVITY | Endogenous |
MW (kDa) | 134 |
Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
Application | Dilution |
---|---|
Western Blotting | 1:1000 |
Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
LARS (D7Q4Q) Rabbit mAb recognizes endogenous levels of total LARS protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro788 of human LARS protein.
Background
Leucyl-tRNA Synthetase (LARS) is a leucine sensor critical for the activation of mTORC1 (1). mTORC1 kinase complex is an important component in the regulation of cell growth (2,3). Its activity is modulated by energy levels, growth factors, and amino acids (4,5). The four related GTPases, RagA, RagB, RagC, and RagD, have been shown to interact with raptor in mTORC1 (2,3). These interactions are both necessary and sufficient for mTORC1 activation in response to amino acid signals (2,3). LARS functions as a GTPase-activating protein (GAP) and interacts directly with RagD GTPase (1). The role of LARS in leucine sensing is not related to its tRNA charging activity (1).
Limited Uses
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