PathScan® RP Phospho-BCKDH-E1α (Ser293) Sandwich ELISA Kit #36390
Important Ordering Details
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Supporting Data
| REACTIVITY | H M R |
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
The PathScan® RP Phospho-BCKDH-E1α (Ser293) Sandwich ELISA Kit detects endogenous levels of BCKDH-E1α protein phosphorylated at Ser293. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Human, Mouse, Rat
Background
Branched-chain amino acids (BCAAs) leucine, isoleucine, and valine are essential amino acids in mammals, but elevated levels of BCAAs have been implicated in cardiovascular and metabolic disorders (1). The branched-chain α-keto acid dehydrogenase complex (BCKDH) catalyzes the rate-limiting step in the BCAA degradation pathway (2,3). Branched-chain α-keto acid decarboxylase (BCKDH-E1) is one of three enzymatic components in this complex (3). The α subunit of BCKDH-E1 (BCKDH-E1α) is critical for the regulation of BCKDH. Phosphorylation of BCKDH-E1α was shown to play a key role in regulating the enzymatic activity of this complex (3-5).
Phosphorylation of BCKDH-E1α at Ser293 inactivates BCKDH (3,4). A significant elevation in plasma BCAA levels was reported to correlate with increased phosphorylation of BCKDH-E1α at Ser293 and suppressed BCKDH activity in the liver of diabetic mice (5).
Limited Uses
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