p115 RhoGEF (D25D2) XP® Rabbit mAb #3669
- WB
- IP
- IF
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 115 |
| Source/Isotype | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
p115 RhoGEF (D25D2) XP® Rabbit mAb detects endogenous levels of total p115 RhoGEF protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human p115 RhoGEF.
Background
The Rho family small GTPases, including Rho, Rac and cdc42, act as molecular switches, regulating processes such as cell migration, adhesion, proliferation and differentiation. They are activated by guanine nucleotide exchange factors (GEFs), which catalyze the exchange of bound GDP for GTP, and inhibited by GTPase activating proteins (GAPs), which catalyze the hydrolysis of GTP to GDP. A third level of regulation is provided by the stoichiometric binding of Rho GDP dissociation inhibitor (RhoGDI) (1).
G-protein coupled receptors (GPCRs) at the cell surface signal through heteromeric G proteins to small GTPases such as Rho, which then signal to downstream effector molecules (2). p115 RhoGEF/ArhGEF1 and its family members PDZ-RhoGEF (PRG), and LARG are stimulated by heteromeric G proteins and thus couple signaling from GPCRs to Rho small GTPases (3-6). In a mouse model of asthma, p115 RhoGEF is necessary for T cells to enable airway inflammation and hyperreactivity (7).
- DerMardirossian, C. and Bokoch, G.M. (2005) Trends Cell Biol 15, 356-63.
- Morris, A.J. and Malbon, C.C. (1999) Physiol Rev 79, 1373-430.
- Chen, Z. et al. (2003) J Biol Chem 278, 9912-9.
- Hart, M.J. et al. (1998) Science 280, 2112-4.
- Kozasa, T. et al. (1998) Science 280, 2109-11.
- Bhattacharyya, R. and Wedegaertner, P.B. (2003) Biochem J 371, 709-20.
- Brown, J.P. et al. (2007) Am J Respir Crit Care Med 176, 10-9.
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