Folate Receptor Alpha/FOLR1 (BN3.2) Mouse mAb #37283
- WB
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 38 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| IHC Leica Bond | 1:50 - 1:200 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
Folate Receptor Alpha/FOLR1 (BN3.2) Mouse mAb recognizes endogenous levels of FOLR1 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to 189 amino acids of the external domain of human FOLR1 protein.
Background
Folate Receptor Alpha, also called FOLR1, is a cysteine-rich cell surface receptor that plays a critical role in the maintenance and transport of folate (vitamin B9) and reduced folic acid derivatives into cells and is essential for various cellular processes (1,2). FOLR1 binds to folate and facilitates its internalization into cells through receptor-mediated endocytosis or potocytosis and is important in cellular processes, including DNA synthesis and repair (1-3). Intracellular folate levels are critical to maintain and support folate functions in its multiple coenzyme forms (4). It is primarily expressed in tissues requiring high folate levels, such as the placenta, kidneys, and certain cancer cells (5). FOLR1 has been found to be overexpressed in several types of cancer, including ovarian, lung, and breast cancer (6-8).
- Chen, C. et al. (2013) Nature 500, 486-9.
- Wibowo, A.S. et al. (2013) Proc Natl Acad Sci USA 110, 15180-8.
- Orr, R.B. and Kamen, B.A. (1994) Cancer Res 54, 3905-11.
- Bailey, L.B. and Gregory, J.F. (1999) J Nutr 129, 779-82.
- Lacey, S.W. et al. (1989) J Clin Invest 84, 715-20.
- Bax, H.J. et al. (2023) Br J Cancer 128, 342-353.
- Nunez, M.I. et al. (2012) J Thorac Oncol 7, 833-40.
- Necela, B.M. et al. (2015) PLoS One 10, e0122209.
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