SDF1/CXCL12 Antibody #3740
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Recombinant protein |
| MW (kDa) | 9 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gln80 of human SDF1/CXCL12. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The stromal cell derived factor 1 (SDF1/CXCL12) is a small, pro-inflammatory chemoattractant cytokine that regulates leukocyte trafficking through interactions with its cognate 7-transmembrane G protein-coupled receptors (1). The SDF1/CXCL12 receptor, CXCR4, also serves as a coreceptor for the entry of human immunodeficiency virus into target cells (2). SDF1/CXCL12 may regulate homing and maintenance of CXCR4-expressing stem or progenitor cells, including embryonic and many somatic stem cells (3,4). Many cancer cells express CXCR4, suggesting that SDF1/CXCL12 plays a role in cancer metastasis (5,6). Alternative splicing and differential processing during maturation produce a pair of SDF1/CXCL12 isoforms (SDF1/CXCL12α and SDF1/CXCL12β) that have different properties and biological activities (7). Additional isoforms of SDF1/CXCL12 have been reported (8,9).
- Maekawa, T. and Ishii, T. (2000) Intern Med 39, 90-100.
- Ray, N. and Doms, R.W. (2006) Curr Top Microbiol Immunol 303, 97-120.
- Sugiyama, T. et al. (2006) Immunity 25, 977-88.
- Ara, T. et al. (2003) Immunity 19, 257-67.
- Kucia, M. et al. (2004) J Mol Histol 35, 233-45.
- Ratajczak, M.Z. et al. (2006) Leukemia 20, 1915-24.
- De La Luz Sierra, M. et al. (2004) Blood 103, 2452-9.
- Gleichmann, M. et al. (2000) Eur J Neurosci 12, 1857-66.
- Yu, L. et al. (2006) Gene 374, 174-9.
Limited Uses
Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.
Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.