MST4 Antibody #3822
- WB
Supporting Data
| REACTIVITY | H M R Mk B |
| SENSITIVITY | Endogenous |
| MW (kDa) | 52 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- B-Bovine
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
MST4 Antibody recognizes endogenous levels of total MST4 protein independent of phosphorylation. The antibody does not cross-react with MST1-3 proteins.
Species Reactivity:
Human, Mouse, Rat, Monkey, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human MST4 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Mammalian sterile-20-like (MST) kinases are upstream regulators of mitogen-activated protein kinase (MAPK) signaling pathways that regulate multiple biological processes, including apoptosis, morphogenesis, cell migration, and cytoskeletal rearrangements (1). This group of serine/threonine kinases includes a pair of closely related proteins (MST1, MST2) that are functionally distinct from the more distantly related MST3 and MST4 kinases. All four MST kinases share a conserved amino-terminal kinase domain and carboxy-terminal regulatory and interaction domains (1-3). At least three of these kinases (MST1-3) promote apoptosis and are activated by caspase cleavage followed by nuclear translocation of the active kinase. MST1/2 kinases play a key role in the Hippo signaling pathway, an evolutionarily conserved program that controls organ size by regulating cell proliferation, apoptosis, and stem cell self renewal (4).
Mammalian Sterile 20-like kinase 4 (MST4, STK26, MASK) is a Golgi-localized kinase that is cleaved by caspase-3 in vitro. While its potential role in apoptosis is unclear, research studies indicate that MST4 is involved in MAPK and EGF pathway signaling (5,6). MST4 and the serine/threonine kinase YSK1 (STK25) localize to the Golgi apparatus following association with the Golgi scaffold protein GM130. Binding to GM130 activates MST4 through autophosphorylation at Thr178 (7).
- Dan, I. et al. (2001) Trends Cell Biol 11, 220-30.
- Creasy, C.L. et al. (1996) J Biol Chem 271, 21049-53.
- Lee, K.K. and Yonehara, S. (2002) J Biol Chem 277, 12351-8.
- Zhao, B. et al. (2011) Nat Cell Biol 13, 877-83.
- Dan, I. et al. (2002) J Biol Chem 277, 5929-39.
- Sung, V. et al. (2003) Cancer Res 63, 3356-63.
- Preisinger, C. et al. (2004) J Cell Biol 164, 1009-20.
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