TPPP/p25 Antibody #39636
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 25 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
TPPP/p25 Antibody recognizes endogenous levels of total TPPP/p25 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu194 of mouse TPPP/p25 protein. Antibodies are purified by peptide affinity chromatography.
Background
Tubulin polymerization-promoting protein (TPPP, also known as p25) is a microtubule nucleation factor in oligodendrocytes. As a largely unstructured protein, TPPP/p25 induces microtubule polymerization in vitro (1,2). TPPP/p25 is enriched in oligodendrocytes in the brain and may play a unique role in the structure and function of these unique cells (3,4). Oligodendrocytes extend elaborate microtubule arbors that contact neuronal axon segments to generate a myelin sheath that is critical for proper propagation of axonal action potentials. TPPP/p25 may not only play a critical role in establishing the microtubule network for oligodendrocytes, but may also contribute to the generation of Golgi outposts, which are Golgi found outside of the cell body. Golgi outposts may play a specialized role in building the local cytoarchitecture of oligodendrocytes and other brain cells (5,6). Misregulation of TPPP/p25 is linked to several neurodegenerative diseases, including Parkinson’s disease, and may contribute to disease etiology and/or represent a potential therapeutic target (7).
- Hlavanda, E. et al. (2002) Biochemistry 41, 8657-64.
- Hlavanda, E. et al. (2007) J Biol Chem 282, 29531-9.
- Takahashi, M. et al. (1991) FEBS Lett 289, 37-43.
- Takahashi, M. et al. (1993) J Neurochem 60, 228-35.
- Fu, M.M. et al. (2019) Cell 179, 132-146.e14.
- Valenzuela, A. et al. (2020) Trends Cell Biol 30, 792-804.
- Oláh, J. et al. (2017) Expert Rev Proteomics 14, 301-309.
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