Phospho-p73 (Tyr99) Antibody #4665
- WB
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr99 of human p73. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The p53 family member, p73, exists in multiple isoforms/splice variants and can induce apoptosis and cell cycle arrest in response to DNA damage via its activity as a transcription regulator (1-3). Upon DNA damage, p73 is phosphorylated at Tyr99 by c-Abl, causing translocation to the nuclear matrix (4). DNA damage-induced acetylation of p73 at Lys321 by the acetyltransferase p300 has also been reported to enhance transcription of genes including that of p53AIP1 (5). Another report, however, indicates that p300 does not acetylate full-length p73 in vivo (6).
While the sequences surrounding p73 Tyr99 and p63 Tyr149 are very similar, only p73 co-localizes with c-Abl following gamma irradiation, suggesting that p63 is not a c-Abl substrate (7).
- Kaghad, M. et al. (1997) Cell 90, 809-19.
- Jost, C.A. et al. (1997) Nature 389, 191-4.
- De Laurenzi, V.D. et al. (1999) Cell Death Differ 6, 389-90.
- Ben-Yehoyada, M. et al. (2003) J Biol Chem 278, 34475-82.
- Costanzo, A. et al. (2002) Mol Cell 9, 175-86.
- Zeng, X. et al. (2001) J Biol Chem 276, 48-52.
- Hamer, G. et al. (2001) Oncogene 20, 4298-4304.
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