FUNDC1 (E2F4T) Rabbit mAb #49240
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 17 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
Storage
Protocol
Specificity / Sensitivity
FUNDC1 (E2F4T) Rabbit mAb recognizes endogenous levels of total FUNDC1 protein. Bands of unknown origin were detected at 45 and 100 kDa.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human FUNDC1 protein.
Background
Mitophagy, a selective form of autophagy targeting damaged mitochondria, is regulated through several protein complexes, including FUNDC1, BNIP3 and BNIP3L/Nix, and PINK1/Parkin (reviewed in 1). FUNDC1 resides on the mitochondrial outer membrane protein and regulates mitochondrial dynamics and hypoxia-induced mitophagy (2-4). It contains an amino-terminal LC3-interacting region (LIR) that is responsible for associating with LC3 and recruiting mitochondria to the autophagosome. The phosphorylation state of FUNDC1 can regulate its activity by affecting its binding to LC3 (4-6). Under normal conditions, FUNDC1 is phosphorylated at Tyr18 by Src, which is inhibited during hypoxia, leading to dephosphorylation (4). FUNDC1 activity is also inhibited by phosphorylation by CK2 at Ser13, which is overcome during hypoxia by the phosphatase PGAM5 which dephosphorylates this site (5). Furthermore, the autophagy kinase ULK1 phosphorylates FUNDC1 at Ser17 which enhances binding to LC3 (6). The levels of FUNDC1 can also be tightly regulated through the ubiquitin/proteasome pathway via the E3 ligase MARCH5, which targets FUNDC1 for degradation (7).
- Pickles, S. et al. (2018) Curr Biol 28, R170-R185.
- Chen, M. et al. (2016) Autophagy 12, 689-702.
- Wu, W. et al. (2016) Autophagy 12, 1675-6.
- Liu, L. et al. (2012) Nat Cell Biol 14, 177-85.
- Chen, G. et al. (2014) Mol Cell 54, 362-77.
- Wu, W. et al. (2014) EMBO Rep 15, 566-75.
- Chen, Z. et al. (2017) EMBO Rep 18, 495-509.
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