PABP1 Antibody #4992
- WB
- IHC
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 71 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:100 |
Storage
Protocol
Specificity / Sensitivity
PABP1 Antibody detects endogenous levels of total PABP1 and PABP3 proteins.
Species Reactivity:
Human, Mouse, Rat, Monkey
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Xenopus, Zebrafish, Bovine
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids at the amino-terminus of human PABP1. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Poly(A)-binding protein 1 (PABP1) associates with the 3' poly(A) tail of mRNA and also eIF4F (1,2). eIF4F is a complex whose functions include the recognition of the mRNA 5' cap structure (eIF4E), delivery of an RNA helicase to the 5' region (eIF4A), bridging of the mRNA and the ribosome (eIF4G), and circularization of the mRNA via interaction between eIF4G and the poly(A) binding protein (PABP). PABP1 has been shown to have multiple functions including translation initiation, mRNA stabilization, and mRNA turnover (3,4). Phosphorylation of PABP has been shown to enhance RNA binding in eukaryotes, and PABP1 has been shown to shuttle between the nucleus and cytoplasm (5,6). PABP1 is methylated on Arg455 and Arg460 by the CARM1 protein methyltransferase (7,8); however, the function of this methylation has yet to be determined.
- Sachs, A. B. et al. (1986) Cell 45, 827-835.
- Piron, M. et al. (1998) EMBO J. 17, 5811-5821.
- Caponigro, G. and Parker, R. (1995) Genes Dev. 9, 2421-2432.
- Sachs, A.B. and Davis, R.W. (1989) Cell 58, 857-867.
- Le, H. et al. (2000) J. Biol. Chem. 275, 17452-17462.
- Afonina, E. et al. (1998) J. Biol. Chem. 273, 13015-13021.
- Lee, J. and Bedford, M.T. (2002) EMBO Rep 3, 268-73.
- Yadav, N. et al. (2003) Proc Natl Acad Sci U S A 100, 6464-8.
Limited Uses
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