IL-1α (D4F3S) Rabbit mAb #50794
- WB
- IP
- F
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 30 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- F-Flow Cytometry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| Flow Cytometry (Fixed/Permeabilized) | 1:200 |
Storage
Protocol
Specificity / Sensitivity
IL-1α (D4F3S) Rabbit mAb recognizes endogenous levels of total IL-1α protein.
Species Reactivity:
Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of mouse IL-1α protein.
Background
Interleukin 1 alpha (IL-1α) belongs to the IL-1 family of cytokines with 11 members including IL-1β. IL-1α is expressed in many cell types of both hematopoietic and non-hematopoietic origins under steady state, and its expression can be increased in response to appropriate stimuli (1,2). Like IL-1β, IL-1α is also synthesized as a precursor (pro-IL-1α) and can be cleaved into smaller mature forms. However, both pro-IL-1α and the cleaved form of IL-1α are biologically active and can activate the signaling pathway through the membrane receptor IL-1R1. IL-1α is active both as a secreted form and as a membrane-bound form. Due to such characteristics, passive leakage of IL-1α from dying cells can activate inflammation, leading some researchers to consider IL-1α as a key “alarmin in the cell” that alerts the host to damage or injury (3,4). In addition, IL-1α can also enter the nucleus to modulate transcription (5,6).
- Garlanda, C. et al. (2013) Immunity 39, 1003-18.
- Palomo, J. et al. (2015) Cytokine 76, 25-37.
- Bertheloot, D. and Latz, E. (2017) Cell Mol Immunol 14, 43-64.
- Di Paolo, N.C. and Shayakhmetov, D.M. (2016) Nat Immunol 17, 906-13.
- Lamacchia, C. et al. (2013) Cytokine 63, 135-44.
- Rider, P. et al. (2013) Semin Immunol 25, 430-8.
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