Cofilin (D3F9) XP® Rabbit mAb #5175
- WB
- IF
Supporting Data
| REACTIVITY | H M R Mk Dg |
| SENSITIVITY | Endogenous |
| MW (kDa) | 19 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
- Dg-Dog
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
Protocol
Specificity / Sensitivity
Cofilin (D3F9) XP® Rabbit mAb detects endogenous levels of total cofilin1 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey, Dog
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to central residues of human cofilin1 protein.
Background
Cofilin and actin-depolymerization factor (ADF) are members of a family of essential conserved small actin-binding proteins that play pivotal roles in cytokinesis, endocytosis, embryonic development, stress response, and tissue regeneration (1). In response to stimuli, cofilin promotes the regeneration of actin filaments by severing preexisting filaments (2). The severing activity of cofilin is inhibited by LIMK or TESK phosphorylation at Ser3 of cofilin (3-5). Phosphorylation at Ser3 also regulates cofilin translocation from the nucleus to the cytoplasm (6).
Limited Uses
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