ECT2 (E7V4L) Rabbit mAb #52021
- WB
Supporting Data
| REACTIVITY | H M Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 104 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
ECT2 (E7V4L) Rabbit mAb recognizes endogenous levels of total ECT2 protein.
Species Reactivity:
Human, Mouse, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro325 of human ECT2 protein.
Background
Epithelial cell transforming sequence 2 (ECT2), a guanine nucleotide exchange factor (GEF), catalyzes the exchange of GDP for GTP. The nuclear GEF activity of ECT2 is essential for Kras-Trp53 lung tumorigenesis. This transformation requires ECT2-mediated rRNA synthesis. In addition, PKCι-dependent phosphorylation of ECT2 is necessary for the oncogenic activity of this protein (1,2). Furthermore, ECT2 has been implicated as a cancer driver gene in certain subtypes of liver cancers (3). Research studies also show that, during matrix-directed cell migration, low membrane tension stimulates the formation of caveolae at the rear end of moving cells. ECT2 is recruited to the caveolae and activates RhoA, causing F-actin organization and rear retraction (4).
Limited Uses
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