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  3. Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free)

Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) #53331

    Product Information

    Product Usage Information

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) contains 10,000 units and is sensitive to denaturation. Mix by gently inverting the tube. Do not vortex. It is recommended to reconstitute with a buffer compatible with the intended assay. Vials should be brought to room temperature prior to opening and they should not be opened in humid areas.

    Storage

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is a lyophilized powder that contains glycine as a stabilizer. Store at 4ºC and protect from moisture. This product is stable for 12 months when stored at 4ºC. Once in solution, store at -20ºC. Aliquot to avoid multiple freeze/thaw cycles.

    Product Description

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free), also known as DNase I, is isolated from bovine pancreas and produced recombinantly in Pichia pastoris. This endonuclease cleaves DNA to produce products with 5'-phospho and 3'-hydroxy ends (1). DNase I is dependent on calcium ions (Ca2+) and activated by magnesium ions (Mg2+) and manganese ions (Mn2+). The Ca2+ work to maintain the enzyme conformation and the Mg2+ and Mn2+ help to catalyze the cleavage of phosphodiester bonds (2). In the presence of Mg2+, DNase I cleaves each strand of dsDNA independently and at random. In the presence of Mn2+, DNase I cleaves each strand of dsDNA at approximately the same site. This enzyme can be used to remove genomic DNA from RNA preparations prior to RT-PCR, to degrade DNA templates after transcription reactions, and to remove unwanted DNA from samples prior to Northern blotting (3-5). DNAse is used in tissue dissociation protocols to digest any DNA that may be present due to cell damage.
    Purity > 99% purity was determined by SDS-PAGE.
    Activity Greater than or equal to 5,000 units per mg protein

    Unit Definition: One unit causes an increase in absorbance at 260 nm of 0.001 per minute at 25ºC when reacting with highly polymerized DNA at pH 5.0 (6).

    Specificity / Sensitivity

    One Kunitz unit digests 1 mg of calf thymus DNA in 10 minutes at 37ºC in 50 mM Tris, 1 mM Mg2+, 1 mM Ca2+, pH 7.8. The correlation of digestion units with Kunitz units can change in other buffer systems.

    Source / Purification

    Deoxyribonuclease I, Recombinant (Animal-Free, Protease and RNase Free) is chromatographically purified from recombinant bovine pancreatic Deoxyribonuclease I produced in Pichia pastoris. Production in yeast decreases levels of contaminating RNase and eliminates potential pathogens associated with animal-based materials.

    Limited Uses

    Except as otherwise expressly agreed in a writing signed by a legally authorized representative of CST, the following terms apply to Products provided by CST, its affiliates or its distributors. Any Customer's terms and conditions that are in addition to, or different from, those contained herein, unless separately accepted in writing by a legally authorized representative of CST, are rejected and are of no force or effect.

    Products are labeled with For Research Use Only or a similar labeling statement and have not been approved, cleared, or licensed by the FDA or other regulatory foreign or domestic entity, for any purpose. Customer shall not use any Product for any diagnostic or therapeutic purpose, or otherwise in any manner that conflicts with its labeling statement. Products sold or licensed by CST are provided for Customer as the end-user and solely for research and development uses. Any use of Product for diagnostic, prophylactic or therapeutic purposes, or any purchase of Product for resale (alone or as a component) or other commercial purpose, requires a separate license from CST. Customer shall (a) not sell, license, loan, donate or otherwise transfer or make available any Product to any third party, whether alone or in combination with other materials, or use the Products to manufacture any commercial products, (b) not copy, modify, reverse engineer, decompile, disassemble or otherwise attempt to discover the underlying structure or technology of the Products, or use the Products for the purpose of developing any products or services that would compete with CST products or services, (c) not alter or remove from the Products any trademarks, trade names, logos, patent or copyright notices or markings, (d) use the Products solely in accordance with CST Product Terms of Sale and any applicable documentation, and (e) comply with any license, terms of service or similar agreement with respect to any third party products or services used by Customer in connection with the Products.

    For Research Use Only. Not For Use In Diagnostic Procedures.
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