RHAMM/CD168 Antibody #55463
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 85 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
RHAMM/CD168 Antibody recognizes endogenous levels of total RHAMM/CD168 protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RHAMM/CD168 protein. The antigenic peptide spans a region that is 100% conserved among the four isoforms of RHAMM/CD168 reported in Uniprot. Antibodies are purified by protein A and peptide affinity chromatography.
Background
Receptor for Hyaluronic acid-Mediated Motility (RHAMM, known also as CD168 or HMMR) was first identified as a putative receptor for hyaluronic acid (HA) that modulated HA-mediated cell motility (1). RHAMM/CD168 is functionally similar to the HA receptor CD44; however in contrast to CD44, RHAMM/CD168 does not contain a transmembrane domain or a signal peptide leader sequence, and so is not targeted exclusively to the cell membrane (1). RHAMM/CD168 has multiple isoforms; some are reportedly exported to the cell membrane in response to signaling by growth factors and cytokines (e.g., TGF-β) (2, 3), whereas others have been implicated in intracellular functions including mitotic spindle regulation (4). Cell surface RHAMM/CD168 is localized to membrane ruffles, consistent with proteins that regulate cell motility (1). Numerous research studies have reported that the expression of RHAMM/CD168 is positively associated with cancer cell growth, motility and/or metastasis (5-7), in addition to HA-mediated inflammation (8), suggesting the potential for therapeutic approaches that target HA-receptor mediated signaling (9,10).
Limited Uses
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