RXRγ Antibody #5629
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 55 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
RXRγ Antibody recognizes endogenous levels of total RXRγ protein. This antibody does not cross-react with either RXRα or RXRβ.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat, Monkey, Bovine, Dog
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RXRγ protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
The human retinoid X receptors (RXRs) are encoded by three distinct genes (RXRα, RXRβ, and RXRγ) and bind selectively and with high affinity to the vitamin A derivative, 9-cis-retinoic acid. RXRs are type-II nuclear hormone receptors that are largely localized to the nuclear compartment independent of ligand binding. Nuclear RXRs form heterodimers with nuclear hormone receptor subfamily 1 proteins, including thyroid hormone receptor, retinoic acid receptors, vitamin D receptor, peroxisome proliferator-activated receptors, liver X receptors, and farnesoid X receptor (1). Since RXRs heterodimerize with multiple nuclear hormone receptors, they play a central role in transcriptional control of numerous hormonal signaling pathways by binding to cis-acting response elements in the promoter/enhancer region of target genes (2).
RXRγ expression, unlike that of RXRα and RXRβ, is restricted largely to cardiac muscle, skeletal muscle, thyroid gland, and thyrotrope cells of the anterior pititary gland (3-6). It is posited that RXRγ plays a pivitol role in the transcriptional control of skeletal muscle differentiation as RXRγ has been found to be directly associated with the promoter regions of MyoD and myogenin (7-10). RXRγ is also involved in the transcriptional control of the hypothalamic-pituitary-thyroid axis through repression of the TSHβ promoter (11-13).
Limited Uses
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