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PDP - Template Name: Monoclonal Antibody
PDP - Template ID: *******c5e4b77
  1. Products /
  2. Primary Antibodies /
  3. DDRGK1 (F6M1S) Rabbit mAb
R Recombinant
Recombinant: Superior lot-to-lot consistency, continuous supply, and animal-free manufacturing.

DDRGK1 (F6M1S) Rabbit mAb #58309

Filter:
  • WB
  • IP

    Supporting Data

    REACTIVITY H
    SENSITIVITY Endogenous
    MW (kDa) 45
    Source/Isotype Rabbit IgG
    Application Key:
    • WB-Western Blotting 
    • IP-Immunoprecipitation 
    Species Cross-Reactivity Key:
    • H-Human 

    Product Information

    Product Usage Information

    Application Dilution
    Western Blotting 1:1000
    Immunoprecipitation 1:100

    Storage

    Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.

    Protocol

    Specificity / Sensitivity

    DDRGK1 (F6M1S) Rabbit mAb recognizes endogenous levels of total DDRGK1 protein. Based on epitope mapping, this antibody is predicted to bind isoform 1 and not isoform 2 of DDRGK1.


    Species Reactivity:

    Human

    Source / Purification

    Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp244 of human DDRGK1 protein.

    Background

    The ubiquitin-fold modifier 1 (UFM1) conjugation system (UFMylation) is a ubiquitin-like system that plays key roles in development and stress responses (1, reviewed in 2-4). Like ubiquitin, conjugation of UFM1 is a three-step enzymatic process involving a specific E1-activating enzyme (UBA5), an E2-conjugating enzyme (UFC1), and an E3-ligation enzyme (UFL1). Several substrates for UFMylation have been identified including DDRGK1, RPL26, p53, MRE11, histone H4, ASC1, CDK5RAP3, and CYB5R3 (5-12). Modification of these targets by UFM1 has direct effects on ER homeostasis, protein translation, and response to DNA damage. Notably, independent screens identified UFMylation as a key regulator of ER-phagy and autophagy (5,13,14). Aberrant UFMylation is associated with many pathological conditions, including cancer, diabetes, and inflammatory diseases (2-4).

    DDRGK1 (also called UFBP1, C20orf116, and Dashurin) is an ER protein that is a key component of the UFMylation system and regulator of ER homeostasis (15,16). It is also a regulator of NF-κB activity (17,18). DDRGK1 is indispensable for embryonic development and hematopoiesis (17).

    1. Tatsumi, K. et al. (2010) J Biol Chem 285, 5417-27.
    2. Gerakis, Y. et al. (2019) Trends Cell Biol 29, 974-986.
    3. Jiang, Q. et al. (2023) Front Endocrinol (Lausanne) 14, 1123124.
    4. Jing, Y. et al. (2022) Cancers (Basel) 14, 3501.
    5. Liu, J. et al. (2017) Nat Commun 8, 14186.
    6. Walczak, C.P. et al. (2019) Proc Natl Acad Sci USA 116, 1299-1308.
    7. Liu, J. et al. (2020) Nat Cell Biol 22, 1056-1063.
    8. Lee, L. et al. (2021) Sci Adv 7, eabc7371.
    9. Qin, B. et al. (2019) Nat Commun 10, 1242.
    10. Yoo, H.M. et al. (2014) Mol Cell 56, 261-274.
    11. Yang, R. et al. (2019) Development 146, dev169235. doi: 10.1242/dev.169235.
    12. Ishimura, R. et al. (2022) Nat Commun 13, 7857.
    13. Liang, J.R. et al. (2020) Cell 180, 1160-1177.e20.
    14. DeJesus, R. et al. (2016) Elife 5, e17290. doi: 10.7554/eLife.17290.
    15. Neziri, D. et al. (2010) Biochim Biophys Acta 1800, 430-8.
    16. Liu, J. et al. (2017) Nat Commun 8, 14186.
    17. Wu, J. et al. (2010) J Biol Chem 285, 15126-15136.
    18. Xi, P. et al. (2013) PLoS One 8, e64231.
    19. Cai, Y. et al. (2015) PLoS Genet 11, e1005643.

    Database Links

    UniProt ID: Q96HY6


    Entrez-Gene Id: 65992


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    For Research Use Only. Not For Use In Diagnostic Procedures.
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