CK1ε (E6K9V) Rabbit mAb #60239
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 43 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
CK1ε (E6K9V) Rabbit mAb recognizes endogenous levels of total CK1ε protein. This antibody does not cross-react with other CK1 proteins.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro352 of human CK1ε protein.
Background
Casein Kinase I (CK1 or CKI) is the name given to a family of kinases consisting of multiple isoforms (α, α', β, γ1-3, δ, and ε) with a conserved N-terminal kinase domain and a variable C-terminal sequence that determines subcellular localization and regulates enzyme activity (1-3). Indeed, multiple inhibitory autophosphorylation sites have been identified near the C terminus of CK1ε (3). This ubiquitously expressed family of protein kinases has been implicated in multiple processes, including DNA repair, cell morphology, and Wnt signaling (4). Perhaps the best understood role of CK1 is to provide the priming phosphorylation of β-catenin at Ser45 to produce the consensus GSK-3 substrate motif (S/T-X-X-X-pS) (4).
CK1ε is involved in many cellular processes, such as differentiation (5-7), cell growth and apoptosis (8), and control of the circadian rhythm (9,10).
- Gross, S.D. and Anderson, R.A. (1998) Cell Signal 10, 699-711.
- Vancura, A. et al. (1994) J Biol Chem 269, 19271-8.
- Gietzen, K.F. and Virshup, D.M. (1999) J Biol Chem 274, 32063-70.
- Polakis, P. (2002) Curr Biol 12, R499-R501.
- Okamura, A. et al. (2004) Blood 103, 2997-3004.
- Swiatek, W. et al. (2006) J Biol Chem 281, 12233-41.
- Bischof, J. et al. (2011) PLoS One 6, e20857.
- Brockschmidt, C. et al. (2008) Gut 57, 799-806.
- Keesler, G.A. et al. (2000) Neuroreport 11, 951-5.
- Meng, Q.J. et al. (2008) Neuron 58, 78-88.
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