GPR52 (E9D8W) Rabbit mAb #61178
- WB
- IF
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 41 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Frozen) | 1:50 - 1:200 |
Storage
For a carrier free (BSA and azide free) version of this product see product #65216.
Protocol
Specificity / Sensitivity
GPR52 (E9D8W) Rabbit mAb recognizes endogenous levels of total GPR52 protein. Nonspecific labeling of Mueller cells in fixed frozen mouse retina has been observed by immunofluorescence.
Species Reactivity:
Human, Mouse
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val249 of human GPR52 protein.
Background
G-protein coupled receptor 52 (GPR52), an orphan G-protein coupled receptor (GPCR), modulates dopaminergic and glutamatergic transmission in neuronal circuits responsible for cognitive function and emotion (1). GPR52 regulates those various brain functions through activation of cAMP-dependent pathways (2). Furthermore, GPR52 is a modulator of soluble mutant huntingtin (mHTT) levels in cells of Huntington’s disease (HD) patients (3). Therefore, GPR52 is a novel therapeutic target for HD as well as psychiatric disorders (1-4). Initial studies have shown that a novel GPR52 antagonist reduces mutant HTT levels and rescues HD-associated phenotypes in cellular and mouse models (4).
Limited Uses
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