NKRF (E9G1T) Rabbit mAb #63409
- WB
- IP
- eCLIP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- eCLIP-eCLIP
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
| eCLIP | 1:200 |
Storage
Protocol
Specificity / Sensitivity
NKRF (E9G1T) Rabbit mAb recognizes endogenous levels of total NKRF protein.
Species Reactivity:
Human, Mouse, Rat
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Dog, Pig
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro75 of human NKRF protein.
Background
NF-κB Repressing Factor (NKRF) is a constitutively expressed transcription factor that binds to the negative regulatory element within the promotor region of many genes that are transactivated by NF-κB (1). Indeed, research studies have found NKRF to repress transcription of IL-8/CXCL8, iNOS, CXCL10, and IFN-γ (2-4). In addition to regulating the expression of pro-inflammatory factors, NKRF has been shown to play a role in cell survival in the context of proteotoxic stress, through the regulation of rRNA processing and ribosome biogenesis (5).
Limited Uses
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