UBR5 (D6O8Z) Rabbit mAb #65344
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 300 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human UBR5 protein.
Background
Human E3 identified by differential display (UBR5/EDD) is a HECT domain-containing ubiquitin E3 ligase of the N-end rule pathway that promotes the ubiquitination and proteasomal degradation of proteins harboring N-degrons (1-3). UBR5 represents an ortholog of HYD, the Drosophila hyperplastic discs tumor suppressor gene product but has been found to be overexpressed in breast and ovarian cancers, suggesting a possible role in promoting tumor development (4,5). Research studies have demonstrated that UBR5 is functional within the nucleus as it participates in DNA damage signaling by controlling the activities of Chk2, TopBP1, and RNF168 (6-9). Recently, UBR5 was shown to play a novel role in immune cell function by regulating RORγt stability and IL-17 production by Th17 cells (10).
- Sriram, S.M. et al. (2011) Nat Rev Mol Cell Biol 12, 735-47.
- Callaghan, M.J. et al. (1998) Oncogene 17, 3479-91.
- Tasaki, T. et al. (2005) Mol Cell Biol 25, 7120-36.
- Clancy, J.L. et al. (2003) Oncogene 22, 5070-81.
- O'Brien, P.M. et al. (2008) Br J Cancer 98, 1085-93.
- Munoz, M.A. et al. (2007) Cell Cycle 6, 3070-7.
- Henderson, M.J. et al. (2006) J Biol Chem 281, 39990-40000.
- Honda, Y. et al. (2002) J Biol Chem 277, 3599-605.
- Gudjonsson, T. et al. (2012) Cell 150, 697-709.
- Rutz, S. et al. (2015) Nature 518, 417-21.
Limited Uses
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