Lamin B1 (119D5-F1) Mouse mAb #68591
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG1 |
Application Key:
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
Lamin B1 (119D5-F1) Mouse mAb recognizes endogenous levels of total Lamin B1 by immunofluroescence. Testing by immunofluorescence was validated for human cells. Reactivity is expected in other species, including mouse and rat.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified rat liver lamins.
Background
Lamins are nuclear membrane structural components that are important in maintaining normal cell functions, such as cell cycle control, DNA replication, and chromatin organization (1-3). Lamins have been subdivided into types A and B. Type-A lamins consist of lamin A and C, which arise from alternative splicing of the lamin A gene LMNA. Lamin A and C are cleaved by caspases into large (41-50 kDa) and small (28 kDa) fragments, which can be used as markers for apoptosis (4,5). Type-B lamins consist of lamin B1 and B2, encoded by separate genes (6-8). Lamin B1 is also cleaved by caspases during apoptosis (9). Research studies have shown that duplication of the lamin B1 gene LMNB1 is correlated with pathogenesis of the neurological disorder adult-onset leukodystrophy (10).
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- Chandler, J.M. et al. (1997) Biochem J 322 ( Pt 1), 19-23.
- Padiath, Q.S. et al. (2006) Nat Genet 38, 1114-23.
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