Phospho-AMPA Receptor 1 (GluA1) (Ser831) (A5O2P) Rabbit mAb #75574
- WB
- IP
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 100 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:50 - 1:250 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Phospho-AMPA Receptor 1 (GluA1) (Ser831) (A5O2P) Rabbit mAb recognizes endogenous levels of AMPA Receptor 1 (GluA1) protein only when phosphorylated Ser831. While the literature refers to this residue as Ser831, it is Ser849 in the UniProt sequence P42261.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser831 of human AMPA Receptor 1 (GluA1) protein.
Background
AMPA- (α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid), kainate-, and NMDA- (N-methyl-D-aspartate) receptors are the three main families of ionotropic glutamate-gated ion channels. AMPA receptors (AMPARs) are comprised of four subunits (GluR 1-4), which assemble as homo- or hetero-tetramers to mediate the majority of fast excitatory transmissions in the central nervous system. AMPARs are implicated in synapse formation, stabilization, and plasticity (1). In contrast to GluR 2-containing AMPARs, AMPARs that lack GluR 2 are permeable to calcium (2). Post-transcriptional modifications (alternative splicing, nuclear RNA editing) and post-translational modifications (glycosylation, phosphorylation) result in a very large number of permutations, fine-tuning the kinetic properties of AMPARs. Research studies have implicated activity changes in AMPARs in a variety of diseases including Alzheimer’s, amyotrophic lateral sclerosis (ALS), stroke, and epilepsy (1).
AMPA-type glutamate receptor activity is regulated by phosphorylation, which plays an important role in synaptic plasticity. CaMKII and PKC phosphorylate GluR 1 at Ser831, while PKA phosphorylates GluR 1 at Ser845 (3-5). Furthermore, Ser845 phosphorylation is increased by activation of D1-type dopamine receptors and by inhibition of protein phosphatase 1/protein phosphatase 2A (5,6). Phosphorylation at either Ser831 or Ser845 potentiates AMPA receptor ion channel function: long-term potentiation (LTP) correlates with increased phosphorylation, while long-term depression (LTD) correlates with a dephosphorylation of GluR 1 (6). Phosphomutant mice (Ser831Ala and Ser845Ala) show deficits in LTD and LTP. Either Ser831 or Ser845 alone may support LTP, while only Ser845 is critical for LTD. Furthermore, these mice have memory deficiencies in spatial learning tasks (7,8). GluR 1 receptors are phosphorylated at either Ser831 or Ser845 at ~15-20% under basal conditions and ~50% under stimulated conditions (behavioral or pharmacological) (9).
- Palmer, C.L. et al. (2005) Pharmacol Rev 57, 253-77.
- Cull-Candy, S. et al. (2006) Curr Opin Neurobiol 16, 288-97.
- Mammen, A.L. et al. (1997) J Biol Chem 272, 32528-33.
- Barria, A. et al. (1997) J Biol Chem 272, 32727-30.
- Roche, K.W. et al. (1996) Neuron 16, 1179-88.
- Lee, H.K. et al. (2000) Nature 405, 955-9.
- Lee, H.K. et al. (2003) Cell 112, 631-43.
- He, K. et al. (2009) Proc Natl Acad Sci U S A 106, 20033-8.
- Diering, G.H. et al. (2016) Proc Natl Acad Sci U S A 113, E4920-7.
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