CD21/CR2 (2G9) Mouse mAb #76069
- IHC
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Mouse IgG2a |
Application Key:
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| IHC Leica Bond | 1:200 - 1:800 |
| Immunohistochemistry (Paraffin) | 1:50 - 1:200 |
Storage
Protocol
Specificity / Sensitivity
CD21/CR2 (2G9) Mouse mAb recognizes endogenous levels of CD21/CR2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a prokaryotic recombinant protein corresponding to the external domain of human CD21/CR2 protein.
Background
Complement receptor type 2 (CD21/CR2) is a type 1 transmembrane glycoprotein whose expression is largely restricted to B lymphocytes and follicular dendritic cells (1,2). Research studies have shown that CD21/CR2 functions to bind the complement fragments iC3b, C3dg, and C3d, which function to activate the alternative complement pathway and MAC formation (3,4). In addition to its function as a complement receptor, CD21/CR2 also functions as the B lymphocyte receptor for Epstein-Barr virus (5) and interferon alpha (6). Research studies have also shown that CD21/CR2 participates in B cell activation, proliferation, and protection from apoptosis through its association with components of the B cell coreceptor signaling complex such as CD19 and CD21 (7-9).
- Nadler, L.M. et al. (1981) J Immunol 126, 1941-7.
- Reynes, M. et al. (1985) J Immunol 135, 2687-94.
- Weis, J.J. et al. (1984) Proc Natl Acad Sci U S A 81, 881-5.
- Nielsen, C.H. et al. (2001) Immunology 104, 418-22.
- Fingeroth, J.D. et al. (1984) Proc Natl Acad Sci U S A 81, 4510-4.
- Asokan, R. et al. (2006) J Immunol 177, 383-94.
- Tedder, T.F. et al. (1994) Immunol Today 15, 437-42.
- Fearon, D.T. and Carter, R.H. (1995) Annu Rev Immunol 13, 127-49.
- Mongini, P.K. et al. (2003) J Immunol 171, 5244-54.
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