IL-1R2 (E3K3O) Rabbit mAb #76888
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60-70 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
IL-1R2 (E3K3O) Rabbit mAb recognizes endogenous levels of total IL-1R2 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domain of human IL-1R2 protein.
Background
IL-1R2 is a type I transmembrane glycoprotein that is expressed predominantly by cells of the myeloid lineage (1). Like IL-1R1, the extracellular domain of IL-1R2 is capable of binding to all three forms of IL-1 (2). Unlike IL-1R1, however, membrane-bound IL-1R2 has a short cytoplasmic tail and does not transduce signals upon binding IL-1 but rather functions as a decoy receptor to dampen IL-1 signaling (3,4). As a negative regulator of the IL-1 receptor signaling complex, research studies have also demonstrated that membrane-bound IL-1R2 undergoes regulated intramembrane proteolysis in response to pro-inflammatory molecules. This process converts the extracellular domain of IL-1R2 into a soluble decoy receptor capable of binding IL-1, limiting its capacity to elicit productive signals through membrane-bound IL-1R1 (5,6). Recent studies have demonstrated that some types of human cancers are enriched with regulatory T cells that express high levels of IL-1R2, an attribute that may contribute to their role in immunosuppression (7,8).
- Colotta, F. et al. (1996) J Immunol 156, 2534-41.
- McMahan, C.J. et al. (1991) EMBO J 10, 2821-32.
- Neumann, D. et al. (2000) J Immunol 165, 3350-7.
- Colotta, F. et al. (1993) Science 261, 472-5.
- Orlando, S. et al. (1997) J Biol Chem 272, 31764-9.
- Uchikawa, S. et al. (2015) Cytokine 71, 238-45.
- Plitas, G. et al. (2016) Immunity 45, 1122-1134.
- De Simone, M. et al. (2016) Immunity 45, 1135-1147.
Limited Uses
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