HMGA2 (D1A7) Rabbit mAb #8179
- WB
- IHC
- IF
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 18 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IHC-Immunohistochemistry
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:200 - 1:800 |
Storage
For a carrier free (BSA and azide free) version of this product see product #44990.
Protocol
Specificity / Sensitivity
Species Reactivity:
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human HMGA2 protein.
Background
HMGA2 belongs to the family of high mobility group with AT-hook DNA binding domain. HMGA proteins are considered architectural transcription factors; they do not have direct transcriptional activation capacity, but instead regulate gene expression by changing DNA conformation through binding to AT-rich regions in the DNA and/or direct interaction with other transcription factors (1,2). HMGA2 is abundantly and ubiquitously expressed and plays a crucial role during embryonic development (3). HMGA2 promotes stem cell self-renewal and research studies have shown that decreased HMGA2 expression is associated with stem cell aging (2,4-6). Investigators have shown that expression levels of HMGA2 are very low in normal adult tissues, while either overexpression or rearrangement is associated with many types of cancer (7-10).
- Cleynen, I. and Van de Ven, W.J. (2008) Int J Oncol 32, 289-305.
- Pfannkuche, K. et al. (2009) Stem Cell Rev 5, 224-30.
- Monzen, K. et al. (2008) Nat Cell Biol 10, 567-74.
- Nishino, J. et al. (2008) Cell 135, 227-39.
- Li, O. et al. (2006) Genesis 44, 523-9.
- Li, O. et al. (2007) FEBS Lett 581, 3533-7.
- Fusco, A. and Fedele, M. (2007) Nat Rev Cancer 7, 899-910.
- Rawlinson, N.J. et al. (2008) Cancer Genet Cytogenet 181, 119-24.
- Wei, J.J. et al. (2010) Am J Surg Pathol 34, 18-26.
- Mahajan, A. et al. (2010) Mod Pathol 23, 673-81.
Limited Uses
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