hnRNP E2/PCBP2 (D1S5E) Rabbit mAb #83017
- WB
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35-45 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
hnRNP E2/PCBP2 (D1S5E) Rabbit mAb recognizes endogenous levels of total hnRNP E2/PCBP2 protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu243 of human hnRNP E2/PCBP2 protein.
Background
PCBP2 (also known as hnRNP E2) is an RNA-binding protein that interacts in a sequence-specific fashion with single-stranded poly (rC). Through their poly(rC)-binding ability, PCBPs regulate mRNA stability and translation (1,2). PCBP2 is an iron chaperone; it delivers iron to ferritin for storage and mediates metalation of certain iron-containing proteins (3-5). PCBP2 interacts with the iron importer DMT1 (divalent metal transporter 1) and the iron exporter FPN1 (ferroportin 1) and regulates their activities (6,7). PCBP2 is induced by viral infection and targets MAVS for polyubiquitination and degradation (8-10). Recent reports demonstrate that it is involved in Hippo signaling, miRNA processing, immune suppression, and cancer (11-15) .
- Choi, H.S. et al. (2009) Biochem Biophys Res Commun 380, 431-6.
- Ren, C. et al. (2016) J Biol Chem 291, 9629-37.
- Leidgens, S. et al. (2013) J Biol Chem 288, 17791-802.
- Nandal, A. et al. (2011) Cell Metab 14, 647-57.
- Frey, A.G. et al. (2014) Proc Natl Acad Sci U S A 111, 8031-6.
- Yanatori, I. et al. (2014) Biochem J 462, 25-37.
- Yanatori, I. et al. (2016) J Biol Chem 291, 17303-18.
- You, F. et al. (2009) Nat Immunol 10, 1300-8.
- Xia, P. et al. (2015) Nat Commun 6, 8132.
- Lee, E.Y. et al. (2016) Nat Immunol 17, 1252-1262.
- Li, F. et al. (2016) Mol Cell Biol 36, 2121-31.
- Li, Y. et al. (2012) Cell Metab 15, 895-904.
- Hu, C.E. et al. (2014) Biochem Biophys Res Commun 448, 437-42.
- Murahashi, M. et al. (2016) Biochem Biophys Res Commun 476, 445-9.
- Chen, X. et al. (2014) Biochem Biophys Res Commun 446, 364-9.
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