PTMScan® LysC Protease #84748
Product Information
Product Usage Information
Storage
Product Description
Specificity / Sensitivity
The activity of LysC is greater than or equal to 1 AU/mg.
Source / Purification
PTMScan® LysC Protease was produced recombinantly in E. coli, purified and provided lyophilized in 1-5% Tris and 20-40% mannitol. Purity was accessed by SDS-PAGE. One amidase unit (AU) of the enzyme produces 1 μmol of p-nitroaniline per min at pH 9.5 at 30°C.
Background
LysC cleaves proteins on the carboxyl side of lysine residues. This makes it a useful tool in protein sequencing and proteomic applications. The enzyme was originally purified from the Gram-negative soil bacteria Achromobacter lyticus (1). It has been found to work optimally between 30-37°C and pH ranges from 9.0-9.5 but will undergo degradation at 50°C and higher. Activity is maintained in 4 M urea or 0.1% SDS solutions for up to 6 hr at 30°C. Inhibitors of the enzyme include PMSF, DFP, and TLCK.
Limited Uses
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