BCL6 (D4I2V) XP® Rabbit mAb (PE Conjugate) #84882
- F
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
BCL6 (D4I2V) XP® Rabbit mAb (PE Conjugate) recognizes endogenous levels of total BCL6 protein. It is predicted to detect both human BCL6 isoform 1 and isoform 2.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a recombinant protein fragment spanning residues Asp210 through Thr409 of human BCL6 protein.
Background
Chromosomal translocations result in misregulation of the proto-oncogene BCL6 in patients with B cell-derived non-Hodgkin's lymphoma (1). The BCL6 gene is selectively expressed in mature B cells and encodes a nuclear phosphoprotein that belongs to the BTB/POZ zinc finger family of transcription factors (2,3). BCL6 protein can bind to target DNA sequences of Stat6 and, analogous to Stat6, modulate the expression of interleukin-4-induced genes (4). Furthermore, BCL6 restrains p53-dependent senescence, making BCL6-active tumors functionally p53-negative (5). The mitogen-activated protein kinases, Erk1 and Erk2, but not JNK, phosphorylate BCL6 at multiple sites. Phosphorylation of BCL6 at Ser333 and Ser343 results in degradation of BCL6 by the ubiquitin/proteasome pathway in B cells (6,7). In addition, BCL6 is acetylated and its transcriptional repressor function is inhibited by the transcriptional co-activator p300 (8).
- Ye, B.H. et al. (1993) Science 262, 747-50.
- Onizuka, T. et al. (1995) Blood 86, 28-37.
- Zollman, S. et al. (1994) Proc Natl Acad Sci U S A 91, 10717-21.
- Harris, M.B. et al. (1999) Mol Cell Biol 19, 7264-75.
- Shvarts, A. et al. (2002) Genes Dev 16, 681-6.
- Moriyama, M. et al. (1997) Oncogene 14, 2465-74.
- Niu, H. et al. (1998) Genes Dev 12, 1953-61.
- Bereshchenko, O.R. et al. (2002) Nat Genet 32, 606-13.
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