FYVE-CENT Antibody #8532
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 285 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
FYVE-CENT Antibody recognizes endogenous levels of total FYVE-CENT protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu595 of human FYVE-CENT protein. Antibodies are purified by protein A and peptide affinity chromatography.
Background
FYVE-CENT (ZFYVE26) contains an FYVE zinc finger domain characterized by conserved R(R/RK) and HHCRxCG motifs (1). Phosphatidylinositol 3 phosphate binds to FYVE-CENT via the FYVE domain and this binding is crucial in phosphoinositide (PtdIns3P)-regulated cytokinesis (1-3). Research evidence suggests that during cytokinesis, FYVE-CENT directly interacts with TTC19 and is recruited to the midbody by the kinesin protein KIF13A (4). FYVE-CENT may also be involved in the tumor suppressor function of Beclin-1 (5). Researchers have linked the defects in FYVE-CENT to hereditary spastic paraplegia, a condition characterized by deterioration of the corpus callosum of the brain (6).
Limited Uses
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