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Render Timestamp: 2024-11-22T12:04:18.484Z
Commit: 5c4accf06eb7154018ba3f54329c7590f97f534a
XML generation date: 2024-11-19 15:05:09.380
Product last modified at: 2024-11-20T08:01:09.644Z
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PDP - Template Name: Buffer
PDP - Template ID: *******ceb613c

PMSF #8553

    Product Information

    Product Usage Information

    For a 200 mM (200X) stock solution, reconstitute the 34.84 mg of lyophilized PMSF supplied in the product tube in 1 mL isopropyl alcohol. PMSF is unstable in water and should be added to lysis buffers or other aqueous solutions just prior to use. Typically, a final concentration of 1 mM provides sufficient protease protection.

    Storage

    Store lyophilized at Room Temperature, protected from direct light. In lyophilized form, the chemical is stable for 24 months. Once in solution, PMSF can be stored at -20° C for up to 3 months, protected from light.

    Product Description

    Phenylmethanesulfonyl Fluoride (PMSF) is an inhibitor of serine proteases such as trypsin, chymotrypsin, thrombin, and papain. It is routinely added as a supplement to lysis buffers just prior to lysis, to prevent protease degradation. Cell Signaling Technology recommends adding PMSF at 1 mM to Cell Lysis Buffer (#9803) and RIPA Buffer (#9806).

    CAS#:

    329-98-6
    MW (kDa) 174.19
    Molecular Formula C7H7FO2S
    For Research Use Only. Not For Use In Diagnostic Procedures.
    Cell Signaling Technology is a trademark of Cell Signaling Technology, Inc.
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