Angiogenesis Antibody Sampler Kit #8696
Product Information
Kit Usage Information
Protocols
- 2478: Western Blotting
- 4060: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 4370: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence*, Flow
- 4511: Western Blotting, Immunoprecipitation (Magnetic), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
- 6943: Western Blotting, Immunoprecipitation (Magnetic)
- 7074: Western Blotting
- 8556: Western Blotting, Immunoprecipitation (Magnetic)
- 8713: Western Blotting, Immunoprecipitation (Agarose), Immunohistochemistry (Paraffin), Immunofluorescence, Flow
Product Description
Specificity / Sensitivity
Each antibody in the Angiogenesis Antibody Sampler Kit recognizes the phosphorylated form of its specific target. Phospho-FAK (Tyr397) (D20B1) Rabbit mAb may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR. Phospho-Src Family (Tyr416) (D49G4) Rabbit mAb may cross-react with other Src family members or overexpressed phosphorylated RTKs. Phospho-VEGFR2 (Tyr1175) (19A10) Rabbit mAb may cross-react with VEGFR1.
Source / Purification
Rabbit monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Ser473 of human Akt protein, Tyr397 of human FAK protein, Thr180/Tyr182 of human p38 MAPK protein, Thr202/Tyr204 of human p44 MAPK protein, Ser1248 of human PLCγ1 protein, Tyr416 of human Src protein, and Tyr1175 of human VEGFR2 protein.
Background
Vascular endothelial growth factor receptor 2 (VEGFR2, KDR, Flk-1) is a major receptor for VEGF-induced signaling in endothelial cells. Upon ligand binding, VEGFR2 undergoes autophosphorylation and becomes activated (1). Signaling from VEGFR2 is necessary for angiogenesis in vivo (2-4). Activation of the receptor leads to rapid recruitment of adaptor proteins, including Shc, GRB2, PI3 kinase, NCK, and the protein tyrosine phosphatases SHP-1 and SHP-2 (5). Phosphorylation of VEGFR2 at Tyr1212 provides a docking site for GRB2 binding and phosphorylation at Tyr1175 binds the p85 subunit of PI3 kinase and PLCγ (1,5,6). Activation of VEGFR2 during angiogenesis leads to signaling through multiple downstream kinase pathways including Akt, Src, FAK, p38, and Erk1/2 (2,7).
- Meyer, M. et al. (1999) EMBO J 18, 363-74.
- Karkkainen, M.J. and Petrova, T.V. (2000) Oncogene 19, 5598-605.
- Rahimi, N. et al. (2000) J Biol Chem 275, 16986-92.
- Claesson-Welsh, L. (2003) Biochem Soc Trans 31, 20-4.
- Holmqvist, K. et al. (2004) J Biol Chem 279, 22267-75.
- Takahashi, T. et al. (2001) EMBO J 20, 2768-78.
- Le Boeuf, F. et al. (2004) J Biol Chem 279, 39175-85.
Database Links
UniProt ID: Q16539 , P27361 , O15264 , P31751 , Q9Y243 , P53778 , P07947 , P07948 , P28482 , P06239 , P19174 , P31749 , P08631 , Q05397 , P12931 , P35968 , P06241 , Q15759
Entrez-Gene Id: 1432 , 5595 , 5603 , 208 , 10000 , 6300 , 7525 , 4067 , 5594 , 3932 , 5335 , 207 , 3055 , 5747 , 6714 , 3791 , 2534 , 5600
Limited Uses
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