RARγ1 (D3A4) XP® Rabbit mAb #8965
- WB
- IP
- IHC
- IF
- F
Supporting Data
| REACTIVITY | H M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
- IF-Immunofluorescence
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
| Immunohistochemistry (Paraffin) | 1:200 - 1:800 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:800 |
| Flow Cytometry (Fixed/Permeabilized) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
RARγ1 (D3A4) XP® Rabbit mAb recognizes endogenous levels of total RARγ1 protein. Based upon sequence alignment, this antibody is not predicted to cross-react with RARγ2. This antibody does not cross-react with either RARα or RARβ.
Species Reactivity:
Human, Mouse
The antigen sequence used to produce this antibody shares 100% sequence homology with the species listed here, but reactivity has not been tested or confirmed to work by CST. Use of this product with these species is not covered under our Product Performance Guarantee.
Species predicted to react based on 100% sequence homology:
Rat, Hamster, Bovine, Dog
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human RARγ1 protein.
Background
Nuclear retinoic acid (RA) receptors (RARs) consist of three subtypes encoded by separate genes: α (NR1B1), β (NR1B2), and γ (NR1B3). For each subtype, there are at least two isoforms, which are generated by differential promoter usage and alternative splicing and differ only in their N-terminal regions. Retinoids, which are metabolites of vitamin A, serve as ligands for RARs (1). RARs function as ligand-dependent transcriptional regulators and are found to be heterodimerized with retinoid X receptors (RXRs). These transcriptionally active dimers regulate the expression of genes involved in cellular differentiation, proliferation, and apoptosis (2,3). Consequently, RARs play critical roles in a variety of biological processes, including development, reproduction, immunity, and organogenesis (4-6). RAR mutations, fusion proteins, altered expression levels, or aberrant post-translational modifications result in multiple diseases due to altered RAR function and disruption of homeostasis.
In contrast to the ubiquitously expressed RARα subtype, RARγ displays a complex tissue-specific expression pattern (7). The hematopoietic system expresses significant levels of RARγ, and a recent study identified a role for RARγ in hematopoietic stem cell maintenance (8). RARγ is the predominant subtype in human and mouse epidermis, representing 90% of the RARs in this tissue (9-11). Given the high level of RARγ expression in the skin, it has been suggested that this nuclear receptor participates in a transcriptional program that governs maintenance and differentiation of normal epidermis and skin appendages. The transcriptional activity of RARγ is under stringent control, in part, through retinoic acid-induced phosphorylation and proteasomal degradation (12).
- Rochette-Egly, C. and Germain, P. (2009) Nucl Recept Signal 7, e005.
- Delacroix, L. et al. (2010) Mol Cell Biol 30, 231-44.
- Eifert, C. et al. (2006) Mol Reprod Dev 73, 796-824.
- Mark, M. et al. (2006) Annu Rev Pharmacol Toxicol 46, 451-80.
- Niederreither, K. and Dollé, P. (2008) Nat Rev Genet 9, 541-53.
- Mark, M. et al. (2009) Nucl Recept Signal 7, e002.
- Dollé, P. (2009) Nucl Recept Signal 7, e006.
- Purton, L.E. et al. (2006) J Exp Med 203, 1283-93.
- Fisher, G.J. et al. (1994) J Biol Chem 269, 20629-35.
- Zelent, A. et al. (1989) Nature 339, 714-7.
- Elder, J.T. et al. (1991) J Invest Dermatol 96, 425-33.
- Giannì, M. et al. (2002) EMBO J 21, 3760-9.
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