CB1 Receptor (D5N5C) Rabbit mAb #93815
- WB
- IP
Supporting Data
| REACTIVITY | H M R |
| SENSITIVITY | Endogenous |
| MW (kDa) | 60 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
CB1 Receptor (D5N5C) Rabbit mAb recognizes endogenous levels of total CB1 Receptor protein.
Species Reactivity:
Human, Mouse, Rat
Source / Purification
Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the extracellular domains of mouse CB1 Receptor protein.
Background
The endocannabinoid system consists of the cannabinoid receptors, CB1 and CB2 receptors, the enzymes that produce and degrade the endogenous cannabinoid ligands (such as FAAH, DAG lipases, and MAG lipase), and the endocannabinoid ligands derived from the metabolism of arachidonic acid, 2-arachidonoylglycerol (2-AG) and anandamide (1-3). CB1 receptor belongs to the superfamily of G protein-coupled receptors (GPCRs) and harbors a large N-terminal extracellular domain, seven transmembrane domains, and a C-terminal intracellular tail. CB1 receptor is coupled to the Gai/o subunit of the G protein which inhibits adenylyl cyclases and regulates calcium and potassium ion channels (4). CB1 receptor is one of the most abundant GPCRs in the central nervous system. It has been show to play critical roles in the wiring of the brain during development (5), in neuronal plasticity (6), analgesia, drug abuse and metabolic homeostasis (4). In addition, CB1 receptor has been shown to interact with other GPCRs, to give rise to novel pharmacological and signaling heteromers with implication in diseases (7,8).
- Devane, W.A. et al. (1992) Science 258, 1946-9.
- Hanus, L. et al. (2001) Proc Natl Acad Sci U S A 98, 3662-5.
- Rozenfeld, R. (2011) Traffic 12, 12-8.
- Mackie, K. (2006) Annu Rev Pharmacol Toxicol 46, 101-22.
- Harkany, T. et al. (2008) Curr Opin Neurobiol 18, 338-45.
- Mackie, K. (2008) Mol Cell Endocrinol 286, S60-5.
- Rozenfeld, R. et al. (2011) EMBO J 30, 2350-63.
- Rozenfeld, R. et al. (2012) PLoS One 7, e29239.
Limited Uses
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