Rab9A (E2I3T) Mouse mAb #94213
- WB
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 23 |
| Source/Isotype | Mouse IgG2b kappa |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
Storage
Protocol
Specificity / Sensitivity
Rab9A (E2I3T) Mouse mAb recognizes endogenous levels of total Rab9A protein. This antibody cross-reacts with nonspecific bands at 60 kDa and 150 kDa.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Rab9A protein.
Background
Rab7 and Rab9 are members of the Ras superfamily of small Rab GTPases (1). Both proteins are located in late endosomes, but exert different functions. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosomes and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). Rab9 interacts with its effector proteins p40 and TIP47 (7,8) to promote the MPR (mannose 6-phosphate receptor)-associated lysosomal enzyme transport between late endosomes and the trans Golgi network (9,10).
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- Feng, Y. et al. (1995) J Cell Biol 131, 1435-52.
- Méresse, S. et al. (1995) J Cell Sci 108 (Pt 11), 3349-58.
- Ceresa, B.P. and Bahr, S.J. (2006) J Biol Chem 281, 1099-106.
- Jäger, S. et al. (2004) J Cell Sci 117, 4837-48.
- Méresse, S. et al. (1999) EMBO J 18, 4394-403.
- Díaz, E. et al. (1997) J Cell Biol 138, 283-90.
- Barbero, P. et al. (2002) J Cell Biol 156, 511-8.
- Lombardi, D. et al. (1993) EMBO J 12, 677-82.
- Riederer, M.A. et al. (1994) J Cell Biol 125, 573-82.
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