EZHIP Antibody #96227
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 80 |
| SOURCE | Rabbit |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
EZHIP Antibody recognizes endogenous levels of total EZHIP protein.
Species Reactivity:
Human
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala495 of human EZHIP protein. Antibodies are purified by peptide affinity chromatography.
Background
EZH inhibitory protein (EZHIP), also called CXorf67 or Catacomb, is a protein discovered in aggressive posterior fossa A ependymomas (1-3). EZHIP contains a highly conserved sequence surrounding a methionine near its C-terminus which mimics the activity of the oncohistone protein H3K27M, and allows it to bind EZH2 and inhibit PRC2 function to lower H3K27me2/3 levels (2-4). EZHIP has also been shown to play a regulatory role in developing sperm and oocytes (5).
Limited Uses
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