Goat Anti-Mouse IgG1, Fc gamma Specific Antibody (HRP Conjugate) #96714
- WB
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | |
| MW (kDa) | |
| Source/Isotype | Goat |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
Product Usage Information
Recommended Antibody Dilutions:
1:3000 - 1:5000
Recommended antibody dilutions are provided in ranges because the optimal dilution is dependent on many factors, such as antigen density and permeability.
Storage
Protocol
Specificity / Sensitivity
Goat Anti-Mouse IgG1, Fc gamma Specific Antibody (HRP Conjugate) detects the Fc portion of mouse IgG1 but does not cross-react with other mouse IgG subclasses, mouse IgM, or the Fab portion of mouse immunoglobulins. This antibody has minimal cross-reaction with human, bovine, and rabbit serum proteins. It may cross-react with immunoglobulins from other species.
Species Reactivity:
Mouse
Source / Purification
Goat Anti-Mouse IgG1, Fc gamma Specific Antibody (HRP Conjugate) is produced by immunizing goats with mouse IgG1. The Anti-Mouse IgG1, Fc gamma Specific Antibody is purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.
Background
Chemiluminescence systems have emerged as the best all-around method for western blot detection. They eliminate the hazards associated with radioactive materials and toxic chromogenic substrates. The speed and sensitivity of these methods are unequalled by traditional alternatives, and because results are generated on film, it is possible to record and store data permanently. Blots detected with chemiluminescent methods are easily stripped for subsequent reprobing with additional antibodies. HRP-conjugated secondary antibodies are utilized in conjunction with specific chemiluminescent substrates to generate the light signal. HRP conjugates have a very high turnover rate, yielding good sensitivity with short reaction times.
Limited Uses
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