PathScan® RP Phospho-HS1 (Tyr378/397) Sandwich ELISA Kit #96948
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Supporting Data
| REACTIVITY | M |
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Description
*Antibodies in this kit are custom formulations specific to kit.
Protocol
Specificity / Sensitivity
The PathScan® RP Phospho-HS1 (Tyr378/397) Sandwich ELISA Kit detects endogenous levels of HS1 protein phosphorylated at Tyr378/397. The kit sensitivity is shown in Figure 1. This kit detects proteins from the indicated species, as determined through in-house testing, but may also detect homologous proteins from other species.
Species Reactivity:
Mouse
Background
HS1 (HCLS1, LckBP1, p75) is a protein kinase substrate that is expressed only in tissues and cells of hematopoietic origin (1,2). HS1 contains four cortactin repeats and a single SH3 domain (2). This intracellular protein is phosphorylated following immune receptor activation, which promotes recruitment of HS1 to the immune synapse (3-5). Phosphorylation of HS1 is required to regulate actin dynamics and provide docking sites for many other signaling molecules, such as Vav1 and PLCγ1 (6). HS1 also plays an important role in platelet activation (7).
HS1 is rapidly phosphorylated at Tyr397 by Syk and/or Lyn kinases following immune receptor stimulation and thrombin-mediated platelet stimulation. This phosphorylation is an important step in cytoskeletal rearrangement and signaling complex formation (6-10).
- Kitamura, D. et al. (1989) Nucleic Acids Res 17, 9367-79.
- Kitamura, D. et al. (1995) Biochem Biophys Res Commun 208, 1137-46.
- Suzuki, H. et al. (1997) J Immunol 159, 5881-8.
- Hata, D. et al. (1994) Immunol Lett 40, 65-71.
- Yamanashi, Y. et al. (1993) Proc Natl Acad Sci USA 90, 3631-5.
- Gomez, T.S. et al. (2006) Immunity 24, 741-52.
- Kahner, B.N. et al. (2007) Blood 110, 2449-56.
- Yamanashi, Y. et al. (1997) J Exp Med 185, 1387-92.
- Hao, J.J. et al. (2004) J Biol Chem 279, 33413-20.
- Brunati, A.M. et al. (2005) J Biol Chem 280, 21029-35.
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