Regucalcin/SMP30 (E3L6L) Rabbit mAb #97025
- WB
- IP
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | 33 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:50 |
Storage
Protocol
Specificity / Sensitivity
Regucalcin/SMP30 (E3L6L) Rabbit mAb recognizes endogenous levels of total regucalcin/SMP30 protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu235 of human regucalcin/SMP30 protein.
Background
Regucalcin is a highly conserved, multifunctional protein encoded by the RGN gene on the X chromosome, generally recognized as a calcium (Ca2+) binding protein involved in the maintenance of intracellular Ca2+ homeostasis (1). The protein was independently identified and termed Senescence Marker Protein-30 (SMP30), following the observation in a rat aging model that expression of the protein markedly declined with age in a hormone-independent manner (2). Regucalcin/SMP30 has since been implicated in the regulatory control of many fundamental biological processes (e.g., cell growth, apoptosis, cellular metabolism), largely through its role in regulating Ca2+ homeostasis and Ca2+-dependent signal transduction (3-5). Research studies also suggest that regucalcin/SMP30 may function as a tumor suppressor in some contexts (3,6), while serum levels of regucalcin/SMP30 have also been proposed as potential biomarkers of liver and kidney dysfunction (7).
- Yamaguchi, M. et al. (1989) Chem Pharm Bull (Tokyo) 37, 388-90.
- Fujita, T. et al. (1992) Biochim Biophys Acta 1116, 122-8.
- Vaz, C.V. et al. (2016) Curr Mol Med 16, 607-19.
- Yamaguchi, M. and Murata, T. (2013) Metabolism 62, 1045-51.
- Yamaguchi, M. (2013) Apoptosis 18, 1145-53.
- Yamaguchi, M. et al. (2017) Mol Cell Biochem 430, 37-46.
- Yamaguchi, M. (2014) Mol Cell Biochem 391, 157-66.
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