ELAVL1/HuR (D9W7E) Rabbit mAb (PE Conjugate) #58289
- F
Supporting Data
| REACTIVITY | H M R Mk |
| SENSITIVITY | Endogenous |
| MW (kDa) | |
| Source/Isotype | Rabbit IgG |
Application Key:
- F-Flow Cytometry
Species Cross-Reactivity Key:
- H-Human
- M-Mouse
- R-Rat
- Mk-Monkey
Product Information
Product Description
Product Usage Information
| Application | Dilution |
|---|---|
| Flow Cytometry (Fixed/Permeabilized) | 1:50 |
Storage
Protocol
Specificity / Sensitivity
ELAVL1/HuR (D9W7E) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total ELAVL1/HuR protein.
Species Reactivity:
Human, Mouse, Rat, Monkey
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His201 of human ELAVL1/HuR protein.
Background
The ELAVL (embryonic lethal, abnormal vision and Drosophila-like) family of proteins includes ELAVL1/HuR, ELAVL2/HuB, ELAVL3/HuC and ELAVL4/HuD (1). ELAVL1/HuR is ubiquitously expressed whereas expression of the other three members is neuronal-specific (1). ELAVL/Hu proteins are highly conserved RNA-binding proteins (1). Besides three RNA recognition motifs, these proteins also contain nuclear localization signals that enable them to shuttle between nucleus and cytoplasm (2). Upon inhibition of transcription by actinomycin D, ELAVL1/HuR relocates from nucleus to cytoplasm where it binds the AU-rich elements within 3' UTRs to stabilize mRNAs (3, 4). ELAVL1/HuR is suggested to increase translation by binding to mRNAs (5,6). In addition, ELAVL1/HuR interacts with microRNAs (miRNAs) (7).
- Izquierdo, J.M. (2008) J Biol Chem 283, 19077-84.
- Fan, X.C. and Steitz, J.A. (1998) Proc Natl Acad Sci U S A 95, 15293-8.
- Peng, S.S. et al. (1998) EMBO J 17, 3461-70.
- Brennan, C.M. and Steitz, J.A. (2001) Cell Mol Life Sci 58, 266-77.
- Mazan-Mamczarz, K. et al. (2003) Proc Natl Acad Sci U S A 100, 8354-9.
- Lal, A. et al. (2005) EMBO J 24, 1852-62.
- Lebedeva, S. et al. (2011) Mol Cell 43, 340-52.
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