CasMINI (8H6-A8) Mouse mAb #53413
- WB
Supporting Data
| REACTIVITY | All |
| SENSITIVITY | Transfected Only |
| MW (kDa) | 70 |
| Source/Isotype | Mouse IgG1 |
Application Key:
- WB-Western Blotting
Species Cross-Reactivity Key:
- All-All Species Expected
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Simple Western™ | 1:10 - 1:50 |
Storage
Protocol
Specificity / Sensitivity
CasMINI (8H6-A8) Mouse mAb recognizes transfected levels of CasMINI protein. This antibody may detect several non-specific bands of unknown origin in rat cell lines.
Species Reactivity:
All Species Expected
Source / Purification
Monoclonal antibody is produced by immunizing animals with full-length recombinant protein specific to CasMINI protein.
Background
CRISPR-Cas (clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins) are RNA-guided nuclease effectors that are utilized for precise genome editing in mammalian systems (1). Cpf1/Cas12a (CRISPR from Prevotella and Francisella) proteins are members of the Class 2 CRISPR system (2). Class 2 CRISPR systems, such as the well characterized Cas9, rely on single-component effector proteins to mediate DNA interference (3). Cpf1/Cas12a and Class 2 CRISPR endonucleases permit efficient and specific genome editing that may be used as gene therapies for genetic diseases. However, virus-based vehicles used for in vivo delivery, like adeno-associated virus (AAV)-based vectors, have a limited packaging capacity. CasMINI was engineered from the naturally occurring type V-F Cas12f (Cas14) system. CasMINI is 529 amino acids, which is significantly smaller than Cpf1/Cas12a and Cas9 proteins (62% and 57% smaller, respectively), making CasMINI more suitable for virus-based packaging for in vivo delivery (4).
Limited Uses
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