CD40 (E2Z7J) Rabbit mAb #86165
- WB
- IP
- IHC
Supporting Data
| REACTIVITY | M |
| SENSITIVITY | Endogenous |
| MW (kDa) | 40 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
- IHC-Immunohistochemistry
Species Cross-Reactivity Key:
- M-Mouse
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:200 |
| IHC Leica Bond | 1:400 |
| Immunohistochemistry (Paraffin) | 1:400 |
Storage
For a carrier free (BSA and azide free) version of this product see product #19554.
Protocol
Specificity / Sensitivity
Species Reactivity:
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val289 of mouse CD40 protein.
Background
CD40, also known as tumor necrosis factor receptor superfamily member 5 (TNFRSF5), is a type I transmembrane protein expressed on the surface of B cells and professional antigen-presenting cells of the immune system, as well as on several non-hematopoietic cell types and cancers (1-4). CD40 interacts with CD40 ligand (CD40L/TNFSF5), which is expressed primarily on activated T cells but has also been reported on blood platelets, mast cells, basophils, NK cells, and B cells (5). Upon engagement with CD40L, CD40 signals through TNF receptor associated factors and MAP kinase signaling pathways, resulting in a wide variety of immune and inflammatory responses, including dendritic cell activation and cross-presentation, T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation (6-8). The CD40/CD40L axis is essential for the initiation and progression of cellular and humoral adaptive immunity, and is an important area of interest in the study of tumor immunology, neurodegenerative diseases, vascular diseases, and inflammatory disorders (9-12).
- Banchereau, J. et al. (1995) Adv Exp Med Biol 378, 79-83.
- Cooke, P.W. et al. (1999) J Pathol 188, 38-43.
- Hakkarainen, T. et al. (2003) Clin Cancer Res 9, 619-24.
- Slobodova, Z. et al. (2011) Neoplasma 58, 189-97.
- Gauchat, J.F. et al. (1993) FEBS Lett 315, 259-66.
- Quezada, S.A. et al. (2004) Annu Rev Immunol 22, 307-28.
- Spriggs, M.K. et al. (1992) J Exp Med 176, 1543-50.
- Elgueta, R. et al. (2009) Immunol Rev 229, 152-72.
- Korniluk, A. et al. (2014) Tumour Biol 35, 9447-57.
- Townsend, K.P. et al. (2005) Eur J Immunol 35, 901-10.
- Hassan, G.S. et al. (2012) Immunobiology 217, 521-32.
- Zhang, B. et al. (2013) Immunol Lett 153, 58-61.
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