CDCA5 (E7M3N) Rabbit mAb #20180
- WB
- IP
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 35 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IP-Immunoprecipitation
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunoprecipitation | 1:100 |
Storage
Protocol
Specificity / Sensitivity
CDCA5 (E7M3N) Rabbit mAb recognizes endogenous levels of total CDCA5 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe247 of human CDCA5 protein.
Background
Cohesin is a ring-shaped multiprotein complex that functions in sister chromatid cohesion and in establishing higher order chromosome structure. The cohesin complex regulates chromosome segregation, DNA repair, and transcription (1,2).
CDCA5 (also known as Sororin) stabilizes the cohesin complex on DNA through inhibition of the cohesion antagonist WAPL (3). CDCA5 promotes cell proliferation and inhibits apoptosis through the AKT signaling pathway in hepatocellular carcinoma (HCC) cells (4). Overexpression of CDCA5 indicates poor prognosis in HCC (5-7).
- Barbero, J.L. (2009) Cell Mol Life Sci 66, 2025-35.
- Waldman, T. (2020) Nat Rev Cancer 20, 504-515.
- Nishiyama, T. et al. (2010) Cell 143, 737-49.
- Chen, H. et al. (2019) J Cancer 10, 1846-1854.
- Tian, Y. et al. (2018) BMC Cancer 18, 1187.
- Wang, J. et al. (2018) Oncol Rep 40, 1875-1884.
- Shen, Z. et al. (2018) Onco Targets Ther 11, 891-901.
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