DPEP-1/MDP (E5C2V) Rabbit mAb #76290
- WB
- IF
Supporting Data
| REACTIVITY | H |
| SENSITIVITY | Endogenous |
| MW (kDa) | 58 |
| Source/Isotype | Rabbit IgG |
Application Key:
- WB-Western Blotting
- IF-Immunofluorescence
Species Cross-Reactivity Key:
- H-Human
Product Information
Product Usage Information
| Application | Dilution |
|---|---|
| Western Blotting | 1:1000 |
| Immunofluorescence (Immunocytochemistry) | 1:400 - 1:1600 |
Storage
Protocol
Specificity / Sensitivity
DPEP-1/MDP (E5C2V) Rabbit mAb recognizes endogenous levels of total DPEP-1/MDP protein. This antibody does not cross-react with either DPEP-2 or DPEP-3 protein.
Species Reactivity:
Human
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro312 of human DPEP-1/MDP protein.
Background
Dipeptidase-1 (DPEP-1, RDP, MDP) is a GPI-linked glycoprotein expressed in the epithelial brush border of the kidney epithelium and exists as a disulfide-linked homodimer (1). Research studies have shown that the enzymatic activity of DPEP-1 is involved in kidney metabolism of glutathione, leukotrienes, and β-lactam antibiotics (2,3). Independent of its enzymatic activity, DPEP-1 has been implicated in pathologic inflammation by acting as an adhesion receptor for neutrophils on endothelial cells within the lung (4). DPEP-1 has also been implicated in human cancer and numerous studies have demonstrated that expression of this enzyme is upregulated in colorectal cancer and promotes metastasis (5-7).
- Hooper, N.M. et al. (1990) Biochem J 265, 429-33.
- Adachi, H. et al. (1989) J Biochem 105, 957-61.
- Campbell, B.J. et al. (1984) J Biol Chem 259, 14586-90.
- Choudhury, S.R. et al. (2019) Cell 178, 1205-1221.e17.
- Tachibana, K. et al. (2017) Biomed Rep 6, 423-428.
- Toiyama, Y. et al. (2011) J Gastroenterol 46, 153-63.
- Park, S.Y. et al. (2016) Oncotarget 7, 9501-12.
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